Keap1 Knockout cell line (LLC)

Loci	STR Info (Sample Cell) Sample Cell Line:LLC				STR Info (Cell bank) Cell Line:LL/2 (LLC1)
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3
18-3	15.0	16.0			15.0	16.0
4-2	21.3	23.3			21.3	23.3
6-7	15.0				15.0
19-2	13.0				13.0
1-2	18.0	19.0			18.0	19.0	20.0
7-1	27.2				26.2	27.2	28.2
8-1	16.0	17.0			16.0	17.0
1-1	15.0	16.0	17.0	18.0	15.0	17.0	18.0
3-2	13.0	14.0			13.0	14.0
2-1	16.0				15.0	16.0
15-3	24.3	25.3	26.3		24.3	25.3	26.3
6-4	18.0				18.0
13-1	18.0				17.0	18.0
11-2	13.3	14.3	15.3		13.3	14.3	15.3
17-2	14.0	15.0			14.0	15.0	16.0
12-1	17.0				17.0
5-5	16.0	17.0			16.0	17.0
X-1	25.0	26.0			25.0	25.3
TH01
D5S818

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Keap1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the LLC cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Keap1 gene knockout Keap1 Knockout cell line (LLC) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Keap1 Knockout cell line (LLC)

Catalog#

YKO-M050

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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Keap1 Knockout cell line (LLC)

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